Table 2 Bacterial strains used in this study
From: Discovery of novel amino acid production traits by evolution of synthetic co-cultures
Strain (abbreviation) | Characteristics | Reference |
|---|---|---|
E. coli |  |  |
DH5α | F−Φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rK− mK+) deoR thi-1 phoA supE44 λ− gyrA96 relA1; strain used for cloning procedures | [61] |
BL21(DE3) | F-ompT hsdSB (rB-, mB-) gal dcm (DE3); host for protein production | [69] |
C. glutamicum |  |  |
ATCC13032 (WT) | Biotin-auxotrophic wild type | [70] |
WT Mrp1CG29D | ATCC13032 with mutation Mrp1CG29D (Cg0325) | This work |
C1* | Derivative of ATCC13032 with a genome reduced by 13.4% | [56] |
C1*ΔLEU ARG+::Ptac-eyfp (ΔLEU ARG+) | C1*ΔLEU ARG+ with eyfp under control of Ptac integrated into the IGR between cg1121 and cg1122 | [9] |
WTΔARG LEU++::Ptac-crimson (ΔARG LEU++) | WTΔARG LEU++ with crimson under control of Ptac integrated in the IGR between cg1121 and cg1122 | [9] |
C1*ΔLEU ARG++ | C1* ΔLEU ARG+ with point mutations ArgBA26V M31V (Cg1582) | [9] |
C1*ΔLEU ARG++::Ptac-eyfp (ΔLEU ARG++) | C1*ΔLEU ARG++ with eyfp under control of Ptac integrated into the IGR between cg1121 and cg1122 | This work |
ΔLEU ARG+ evo1 | Derivative of ΔLEU ARG+ isolated after evolution. For details about identified mutations, see Table 1 | This work |
ΔLEU ARG+ evo2 | Derivative of ΔLEU ARG+ isolated after evolution. For details about identified mutations, see Table 1 | This work |
ΔARG LEU++ evo1 | Derivative of ΔARG LEU++ isolated after evolution. For details about identified mutations, see Table 1 | This work |
ΔARG LEU++ evo2 | Derivative of ΔARG LEU++ isolated after evolution. For details about identified mutations, see Table 1 | This work |
ΔARG LEU++ evo3 | Derivative of ΔARG LEU++ isolated after evolution. For details about identified mutations, see Table 1 | This work |
C1*ΔLEU ARG+::Ptac-eyfp MetC/PbrnQ* (ΔLEU ARG+ MetC/PbrnQ*) | C1*ΔLEU ARG+::Ptac- eyfp with mutation MetCS322F (Cg2536), which is also within the promoter region of brnQ (cg2537) | This work |
C1*ΔLEU ARG+::Ptac-eyfp Mrp1CG29D (ΔLEU ARG+ Mrp1CG29D) | C1*ΔLEU ARG+::Ptac- eyfp with mutation Mrp1CG29D (Cg0325) | This work |
C1*ΔLEU ARG+ Mrp1AH335P (ΔLEU ARG+ Mrp1AH335P) | C1*ΔLEU ARG+::Ptac- eyfp with mutation Mrp1AH335P (Cg0326) | This work |
C1*ΔLEU ARG+::Ptac-eyfp MetC/PbrnQ* Mrp1CG29D (ΔLEU ARG+ MetC/PbrnQ* Mrp1CG29D) | C1*ΔLEU ARG+::Ptac- eyfp MetC/PbrnQ* with mutation Mrp1CG29D (Cg0325) | This work |
C1*ΔLEU ARG+::Ptac-eyfp Δmrp1 (ΔLEU ARG+ Δmrp1) | C1*ΔLEU ARG+::Ptac- eyfp with deletion of mrp1 (cg0321-cg0326) and cg0317-cg0319. The latter encode genes for arsenate/arsenite resistance and were deleted accidentally | This work |
WTΔARG LEU++::Ptac-crimson PargT*1 (ΔARG LEU++ PargT*1) | WTΔARG LEU++::Ptac-crimson with mutation A ➜ G 35 bp upstream of the argT (cg1504) TSS/TLS | This work |
WTΔARG LEU++::Ptac-crimson argT* (ΔARG LEU++ argT*) | WTΔARG LEU++::Ptac-crimson with mutation of the 3rd codon of argT (cg1504) GAG ➜ GAA, synonymous mutation | This work |
WTΔARG LEU++::Ptac-crimson PargT*1 Cg1874G93D (ΔARG LEU++ PargT*1 Cg1874G93D) | WTΔARG LEU++::Ptac-crimson PargT*1 with mutation Cg1874G93D | This work |
WTΔARG LEU++::Ptac-crimson PargT*1 Cg1874G93D Cg2850G30R (ΔARG LEU++ PargT*1 Cg1874G93D Cg2850G30R) | WTΔARG LEU++::Ptac-crimson PargT*1 Cg1874G93D with mutation Cg2850G30R | This work |
WTΔARG LEU++::Ptac-crimson ΔargTUV (ΔARG LEU++ ΔargTUV) | WTΔARG LEU++::Ptac-crimson with in frame deletion of argTUV (cg1504-1502) | This work |
WTΔARG LEU++::Ptac-crimson Δcg1505-1506 (∆ARG LEU++ ∆cg1505-cg1506) | WTΔARG LEU++::Ptac-crimson with deletion of cg1505 and cg1506 including their promoters | This work |
WT∆LEU ARG−+::Ptac-eYFP (∆LEU ARG−+) | WT with an in-frame deletion of ΔleuA (cg0303), ΔleuC (cg1487), ΔleuD (cg1488), ΔleuB (cg1453), with point mutations ArgBA26V M31V (Cg1582) and eyfp under control of Ptac integrated into the IGR between cg1121 and cg1122 | This work |