Discovery of novel amino acid production traits by evolution of synthetic co-cultures

Table 1 Mutations identified by genome sequencing in evolved CoNoS strains

Strain	Position & mutation on DNA level^a	Locus tag	Mutation	Mutation designation
∆LEU ARG⁺ evo1	SNV C281573T	cg0325	Mrp1C_G29D	Mrp1C_G29D
∆LEU ARG⁺ evo1	SNV C2044002T	cg2536/P_cg2537	MetC_S322F/P_brnQ*	MetC/P_brnQ*
∆LEU ARG⁺ evo2	SNV T283546G	cg0326	Mrp1A_H335P	Mrp1A_H335P
∆LEU ARG⁺ evo2	SNV C2044002T	cg2536/P_cg2537	MetC_S322F/P_brnQ*	MetC/P_brnQ*
∆ARG LEU⁺⁺ evo1	SNV T1399043C	P_cg1504	P_cg1504*¹, mutation of -35 region: ttaagg ➜ ttgagg	P_argT*¹
	SNV G1767718A	cg1874	Cg1874_G93D	Cg1874_G93D
	SNV G2712428C	cg2850	Cg2850_G30R	Cg2850_G30R
∆ARG LEU⁺⁺ evo2	DEL 1399026–1401854	P_cg1504, cg1505, cg1506	deletion of 2829 bp upstream of cg1504^b	P_argT*²
∆ARG LEU⁺⁺ evo2	SNV G1767718A	cg1874	Cg1874_G93D	Cg1874_G93D
∆ARG LEU⁺⁺ evo3	SNV C1399000T	cg1504	cg1504*, 3rd codon GAG ➜ GAA, synonymous mutation	argT*
	SNV G1767718A	cg1874	Cg1874_G93D	Cg1874_G93D
	SNV G2712428C	cg2850	Cg2850_G30R	Cg2850_G30R

^aReads were mapped using GenBank accession number CP017995 (for ∆LEU ARG⁺) or BX927147 (for ∆ARG LEU⁺⁺) as reference. Mutations are given for the plus strand. Abbreviations: deletion (DEL); single nucleotide variant (SNV)
^bPartial deletion of the intergenic region of cg1504-cg1505, deletion of cg1505 & cg1506, partial deletion of cg1507

ISSN: 1475-2859