Fig. 6
From: Discovery of novel amino acid production traits by evolution of synthetic co-cultures
Characterization of ArgTUV as l-arginine importer. A Comparison of ∆ARG LEU++ ΔargTUV to the parental strain and the WT during growth in BHI medium. All strains were precultivated in BHI medium. B Comparison of ∆ARG LEU++ ΔargTUV to the parental strain and the WT in CGXII with 2% (w/v) glucose supplemented with l-arginine or dipeptides as indicated. Strains marked with * did not grow. The WT monoculture without amino acid supplementation is shown as reference. ∆ARG LEU++ ΔargTUV was pre-cultivated in BHI, all other strains were pre-cultivated in CGXII medium with 2% (w/v) glucose and 3 mM of the required amino acid. A, B Backscatter data were normalized by the maximum value recorded for the WT monoculture. Mean values and standard deviations of three biological replicates are shown as lines and shaded areas, respectively. C Raw data of an ITC experiment with 200 µM l-arginine and 30 µM His10-ArgT in 40 mM HEPES–NaOH buffer, pH 7.4, with 100 mM NaCl. D Corresponding binding isotherm created by plotting the integrated heat peaks against the molar ratio