Fig. 3From: Discovery of novel amino acid production traits by evolution of synthetic co-culturesGrowth performance of Mrp1 mutant strains in supplemented monocultures under NaCl stress conditions. A Monocultures of WT and WT Mrp1CG29D cultivated with 2% (w/v) glucose. B Monocultures of mutated ∆LEU ARG+ strains in comparison to WT, ∆LEU ARG+. For (A, B) cultures were performed in biological triplicates in CGXII medium with 2% (w/v) glucose, supplemented with 3 mM l-leucine for auxotrophic strains. Mean values and standard deviations are shown as lines and shaded areas, respectively. C Tenfold serial dilutions of different C. glutamicum Mrp1 mutant strain cultures. Cells were precultivated in test tubes at 30 °C 180 rpm for 8 h in BHI medium. Afterwards, second precultures were prepared in CGXII with 2% (w/v) glucose and 3 mM l-leucine for the auxotrophic strains and cultivated over night at 30 °C 180 rpm. Dilutions were prepared in PBS starting with an OD600 of 1 and spotted onto CGXII-agar plates (pH 8.0, 2% (w/v) glucose, 3 mM l-leucine) with different NaCl concentrations (0 M and 0.6 M) and incubated at 30 °C for 48 h, as described elsewhere [25]Back to article page