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Table 1 Bacterial strains and plasmids used for this study

From: Development of high cell density Limosilactobacillus reuteri KUB-AC5 for cell factory using oxidative stress reduction approach

Strains and plasmids

Relevant features

Source

Strains

 Escherichia coli

  MC1061

Cloning host recA+

MoBiTec

Limosilactobacillus reuteri

 KUB-AC5

Probiotic lactic acid bacteria

Lab stock

 pSIP411 + kat

KUB-AC5 carrying catalase gene in pSIP411 expression plasmid

This study

 pSIP411 + sod

KUB-AC5 carrying superoxide dismutase gene in pSIP411 expression plasmid

This study

Plasmids

 pSIP411gusA

spp-based expression vector, PSIP401 derivative, gusA controlled by PsppQ (PorfX), sppKR expression driven by eryB read through, with SH71rep

[44, 45]

 pSIP411 + kat

ery, pSIP401 derivative, Mn-catalase gene for L. reuteri, with SH71rep

This study

 pSIP411 + sod

ery, pSIP401 derivative, Mn-superoxide dismutase gene for L. reuteri, with SH71rep

This study