Fig. 5

Expression of the small laccases genes in B. subtilis RIK1285 host organism. Secretion of a) ScLac, b) SvLac, c) ScLac ∆SP and d) AmLac ∆SP. Bacteria were inoculated in the growth media containing 100 µM of CuSO₄. The samples were taken at defined time points, cells were separated by centrifugation and activity was measured using ABTS as a substrate. The bacterial growth was measured spectrophotometrically at 600 nm. ScLac and SvLac contained signal sequences at the N terminus, whereas in AmLac ∆SP and ScLac ∆SP the signal sequence was deleted. ScLac and SvLac were secreted with their signal sequence still attached. ScLac ∆SP and AmLac ∆SP were secreted without signal sequences. The activity results were the average of three biological replicates in three technical replicates each