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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Fig. 4

Alkalinization of the medium triggers an oxidative stress response in K. phaffii. (A) Changes in expression for diverse genes known to be responsive to oxidative stress in yeasts. Data corresponds to cells grown in glycerol and switched to pH 8.2. The discontinuous red line indicates, in this and subsequent figures, the log2 > = 1 and <= -1 thresholds. (B) K. phaffii X33 cells were grown in YPD medium and incubated for 1 h with dihydrorhodamine 123. Aliquots were taken and treated with KCl (30 mM), or KOH (20 and 30 mM, raising the pH to 7.4 and 8,1, respectively). After 30 min of incubation, cells were processed for flow cytometry. The lower panel shows the fluorescence shift of the population treated with 30 mM KOH. (C) Cultures treated with 30 mM KOH) were observed under a Nikon Eclipse TE2000-E using the FIT-C filter. Scale bar denotes 5 μm

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