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Fig. 10 | Microbial Cell Factories

Fig. 10

From: Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Fig. 10

Putative CDRE elements are crucial for pH-dependent induction of PHO89 expression. (A) Putative CDRE elements were identified in the PHO89 promoter (CDRE 1 and CDRE 2). (B) The constant GCC core was mutated individually or in combination (CDRE 1 + 2) and the response of cells bearing PHO89-GFP reporters carrying such mutations was compared by flow cytometry with that of the native version (denoted as PHO89). Data is represented as the mean ± SEM of the fold-change in comparison with unstressed cells from 4 experiments. (C) Cells bearing the reporters indicated above were incubated for 8 h with 0.6 M KCl or CaCl2 and samples taken at t = 0 and 8 h for flow cytometry analysis. Values were compared with cells incubated with KCl to avoid interference of possible osmotic effects. Data is the mean ± SEM of the fold-change from 3 or 4 experiments. *, p < 0.05; **, p < 0.01; ns, not significant (p > 0.05)

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