Fig. 1
Schematic representation of ALE of Yarrowia lipolytica. Three ALE strategies were designed to increase biomass and lipid formation under high pure and crude glycerol concentrations. After 104 passages, evolved populations were cryo-preserved and evolved single colonies were isolated. WTpar, Y. lipolytica 28849 parental strain; EMSmut, after random chemical mutagenesis with ethyl methanesulfonate (EMS) exposure