Fig. 5
From: Engineering Escherichia coli for efficient assembly of heme proteins
Characterization of B. subtilis dye-decolorizing peroxidase (BsDyp) purified from E. coli BL21(DE3) and Ec-M13 strains. A The Rz (A407/A280) ratio of purified BsDyp protein. B The enzyme activity of purified BsDyp protein. Exo-heme, exogenously supplemented with 0, 1, 5, 10 or 20Â uM heme; Chaperones, co-expression of molecular chaperones including GroES, GroEL and Tig; M1 & M3 modules, overexpression of the 7 genes involved in module 1 and module 3 (Ec-M13 strain)