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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Engineering Escherichia coli for efficient assembly of heme proteins

Fig. 2

Characterization of fermentation products of heme-producing K. phaffii strains. A The fermentation cultures of GS115, pPH4 and pPH8 strains. B HPLC analysis of fermentation extract of pPH8 strain and heme standard (heme std) at 400 nm. C ESI–MS/MS chromatogram analysis of heme produced by pPH8 (indicated by the arrow in B and heme std. D ESI ( +) EIC analysis of fermentation products of pPH8 strain. E ESI–MS/MS chromatogram analysis of chemical standards including porphobilinogen (C10H14N2O4), hydroxymethylbilane (C40H46N4O17), coproporphyrinogen III (C36H44N4O8), protoporphyrinogen IX (C34H40N4O4) and protoporphyrin IX (C34H34N4O4). F ESI–MS/MS chromatogram analysis of fermentation product of pPH8 strain indicated by the corresponding arrows in D

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