Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures

Fig. 4

The effects of exogenous BCAAs (l-Ile, l-Val and l-Leu) on growth and perylenequinone production of S. bambusicola S8. A The effects on red pigments secretion of S. bambusicola S8 in PDA plates. B The effects on perylenequinone production in plate. The fungus was treated with BCAAs (1.5 g/L) and incubated at 28 °C for 8 days. C The spore germination rate. The spores were washed and cultured in a 30% PDB medium containing l-Val or l-Leu (1.5 g/L). D Conidium concentration of S8 were measured on day 8. E The mycelial morphology was observed (200 ×). F Determination of the length of hyphae branching. A small lump (4 × 4 mm) of S. bambusicola S8 was transferred from stock slants to petri dishes containing l-Val and l-Leu (1.5 g/L), and then incubated at 28 °C for 3 days. The sterile cover glass was inserted into the edge of the hyphae, and the morphology of the hyphae was observed after 2 days of culture. The culture without l-Val or l-Leu treatment was used as control. Values are mean ± SD from three independent experiments (*p < 0.05, **p < 0.01 versus control group)

Back to article page