Fig. 5

Application of mechanosensitive knockout for osmolysis in E. coli BL21. A Percent cell lysis following growth in LB with 4% NaCl of BL21 (blue), BL21 ΔmscL (red), and BL21 ΔmscL ΔmscS (yellow) in three different media: commercial B-PER™ Bacterial Protein Extraction Reagent; a 4% NaCl_(aq) isotonic solution; and distilled water (diH2O). Cell lysis is determined as the fraction of RFP recovered in supernatant compared to total RFP content, as it was in C. necator, with concentration measured by fluorescence intensity (n = 6). B Growth rate (red diamonds) and osmolysis efficiency (blue circles) of BL21 ΔmscL ΔmscS in LB with various (final) NaCl concentrations (n = 3). C SDS-PAGE gel of various fractions of RFP-expressing BL21 and BL21 ΔmscL ΔmscS. NE: cells not expressing RFP, WC: whole-cell fraction, S: post-osmolysis supernatant fraction, P: post-osmolysis cell pellet. For each data point in (A) and (B), mean ± SD is displayed