Fig. 4
Effect of combined mscL gene knockout and improved halotolerance on osmolysis in C. necator. Osmolysis efficiency, calculated as a fraction of RFP recovered in cell-free supernatant compared to total RFP content following osmotic shock (relative concentrations determined by fluorescence intensity), as a function of osmotic downshock magnitude for ht030b (blue circles) and ht030b ΔmscL (red diamonds) following growth in (A) LB with 3.0% NaCl (w/v, final concentration) and (B) M9 sodium formate (4 g/L) medium supplemented with 16 g/L NaCl. Differences in values on the x-axis of the two graphs reflect differences in starting osmolarities of the two media tested. For each data point, mean ± SD is displayed (n = 6)