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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products

Fig. 3

Osmolysis efficiency, calculated as a fraction of RFP recovered in supernatant compared to total RFP content following osmotic shock (see methods), as a function of osmotic downshock magnitude for wild-type C. necator H16 (blue circles) and mutant C. necator H16 ΔmscL (red diamonds) following growth in (A) LB with 1.5% (w/v) NaCl (final concentration) and (B) M9 sodium formate (4 g/L) medium supplemented with 6 g/L NaCl. Differences in values on the x-axis of the two graphs reflect slight differences in starting osmolarities of the two media tested. For each data point, mean ± SD is displayed (n = 6)

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Microbial Cell Factories

ISSN: 1475-2859

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