Table 2 Central Composite Design along with experimental and predicted responses for PHA production by Cupriavidus sp. CB15
Standard order | Factors | Response 1 | Predicted Response 1 | Response 2 | Predicted Response 2 | Response 3 | Predicted Response 3 | ||
|---|---|---|---|---|---|---|---|---|---|
NH4Cl (g/L) | Temperature (°C) | Shaking speed (rpm) | DCW (g/L) | DCW (g/L) | PHA production (g/L) | PHA production (g/L) | PHA content (wt%) | PHA content (wt%) | |
1 | 0.05 | 40.00 | 125.00 | 0.22 | 0.27 | 0.15 | 0.19 | 68.0 | 53.0 |
2 | 1.50 | 40.00 | 125.00 | 2.06 | 2.34 | 0.93 | 1.17 | 45.5 | 53.7 |
3 | 0.05 | 50.00 | 125.00 | 0.41 | 0.45 | 0.09 | 0.10 | 22.1 | 16.4 |
4 | 1.50 | 50.00 | 125.00 | 0.37 | 0.51 | 0.15 | 0.22 | 40.3 | 36.0 |
5 | 0.05 | 40.00 | 225.00 | 0.40 | 0.48 | 0.15 | 0.17 | 37.4 | 35.6 |
6 | 1.50 | 40.00 | 225.00 | 2.83 | 3.01 | 1.14 | 1.23 | 40.2 | 39.9 |
7 | 0.05 | 50.00 | 225.00 | 0.01 | − 0.05 | 0 | − 0.14 | 0 | − 14.3 |
8 | 1.50 | 50.00 | 225.00 | 0.30 | 0.47 | 0 | 0.05 | 0 | 9.0 |
9 | 0 | 45.00 | 175.00 | 0.03 | 0.08 | 0 | 0.09 | 0 | 19.1 |
10 | 1.99 | 45.00 | 175.00 | 2.61 | 2.26 | 1.30 | 1.08 | 49.7 | 39.2 |
11 | 0.78 | 36.59 | 175.00 | 2.17 | 1.93 | 1.32 | 1.13 | 60.6 | 63.0 |
12 | 0.78 | 53.41 | 175.00 | 0 | − 0.06 | 0 | 0.06 | 0 | 6.2 |
13 | 0.78 | 45.00 | 90.91 | 1.14 | 0.94 | 0.47 | 0.30 | 41.4 | 48.4 |
14 | 0.78 | 45.00 | 259.09 | 1.19 | 1.08 | 0.11 | 0.15 | 9.5 | 11.0 |
15 | 0.78 | 45.00 | 175.00 | 2.57 | 2.72 | 1.84 | 1.98 | 71.6 | 72.3 |
16 | 0.78 | 45.00 | 175.00 | 2.81 | 2.72 | 2.09 | 1.98 | 74.4 | 72.3 |