Table 1 Strains and plasmids used in this study

Strain

 S.albulus

  WG608

Parent strain, ε-poly-L-lysine producer

[29]

  PL01

WG608 carrying pIB139-pap

This study

  PL02

WG608 carrying pIB139-ppk1

This study

  PL03

WG608 carrying pIB139-ppk2B^cg

This study

  PL04

WG608 carrying pIB139-ppk2C^pa

This study

  PL05

WG608 carrying pIB139-ppk2B^cg-pap

This study

  PL06

WG608 carrying pIB139-ppk2B^cg-pap-ppk1

This study

 E. coli

  DH5α

General cloning host

Invitrogen

  ET12567

Donor strain for conjugation between E.coli and Streptomyces

Invitrogen

  BL21(DE3)

Host for expression of PPKs

Invitrogen

  BL21-pET28a-ppk1

BL21 carrying pET28a-ppk1

This study

  BL21-pET28a-ppk2B^cg

BL21 carrying pET28a-ppk2B^cg

This study

  BL21-pET28a-ppk2C^pa

BL21 carrying pET28a-ppk2C^pa

This study

 Plasmids

  pIB39

Integrative vector based on ϕC31 integrase

[30]

  pIB139-ppk1

ppk1 cloned in pIB139

This study

  pIB139-ppk2B^cg

ppk2B^cg cloned in pIB139

This study

  pIB139-ppk2C^pa

ppk2C^pa cloned in pIB139

This study

  pIB139-pap

pap cloned in pIB139

This study

  pIB139-ppk2B^cg-pap

ppk2B^cg and pap cloned in pIB139

This study

  pIB139-ppk2B^cg-pap-ppk1

ppk2B^cg, pap and ppk1 cloned in pIB139

This study

  pET28a( +)

E. coli expression vector

Novagen

  pET28a-ppk1

ppk1 cloned in pET28a( +)

This study

  pET28a-ppk2B^cg

ppk2B^cg cloned in pET28a( +)

This study

  pET28a-ppk2C^pa

ppk2C^pa cloned in pET28a( +)

This study