Table 2 Strains used in this study
Strains | Description | Source |
|---|---|---|
E. coli | ||
BL21-kd1/kd2/kd3/kd4/kd5 | Recombinant BL21 (DE3) cells, possessing pET28a-kstD1/kstD2/kstD3/kstD4/ kstD5 | This study |
Mycobacterium fortuitum | ||
ATCC 35855 | 9-OH-AD as a main product during sterol consumption and degradation with increasing fermentation time | ATCC |
MFΔkd1/2/3/4/5 | kstD1/2/3/4/5 deleted mutant of ATCC 35855 | This study |
MFΔkd23 | kstD2&3 deleted mutant of ATCC 35855 | This study |
MFΔkd123 | kstD1&2&3 deleted mutant of ATCC 35855 | This study |
MFΔkd1234 | kstD1&2&3&4 deleted mutant of ATCC 35855 | This study |
MFΔkstD | kstD1&2&3&4&5 deleted mutant of ATCC 35855 | This study |
MFΔkstD-0 | MFΔkstD carrying a vacant p40 | This study |
MFΔkstD-1/2/3/4/5 | kstD1/2/3/4/5 over-expression in MFΔkstD via p40-kstD1/2/3/4/5 | This study |
MFΔkstD_hsd4A | hsd4A from M. neoaurum DSM 44074 over-expression in MFΔkstD via p40-Hsd4A | This study |
MFΔkstDΔopccr | opccr deleted mutant of MFΔkstD | This study |
MFΔkstDΔopccr_hsd4A | hsd4A from M. neoaurum DSM 44074 over-expression in MFΔkstDΔopccr via p40-Hsd4A | This study |
MFΔkstDΔopccrΔfadE26-27 | fadE26-27 deleted mutant of MFΔkstDΔopccr | This study |
MFΔkstDΔopccr ΔfadE28-29 | fadE28-29 deleted mutant of MFΔkstDΔopccr | This study |
MFΔkstDΔopccr_ fadE28-29 | fadE28-29 from over-expression in MFΔkstDΔopccr via p40-60 | This study |
MF-FA5020 | hsd4A and fadE28-29 from over-expression in MFΔkstDΔopccr via p40-60 | This study |