Fig. 5From: Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-Δ1-dehydrogenase deletions and multiple genetic modifications in Mycobacterium fortuitumPhenotypic analyses of M. fortuitum ATCC 35855 and mutants. A Growth curve of M. fortuitum ATCC 35855; B time course of phytosterols degradation and 9-OHAD accumulation in M. fortuitum ATCC 35855; C HPLC of phytosterol degradation at 48 h by M. fortuitum ATCC 35855; D–F 9-OHAD accumulation of single or multiple kstDs-deleted mutants and single kstD complemented strains in the medium MF/01 supplemented with 10 g/l phytosterol. Standard deviations of the biological replicates are represented by error barsBack to article page