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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Enhancement of herbicolin A production by integrated fermentation optimization and strain engineering in Pantoea agglomerans ZJU23

Fig. 6

Optimization of HA production increased the biocontrol efficacy against gray mold caused by a pyrimethanil resistant strain. a Mycelial growth inhibition of pyrimethanil and HA towards B. cinerea (B.c) pyrimethanil-sensitive strain B05.10 and pyrimethanil-resistant strain Pyr-R5. The final concentration of pyrimethanil or HA was 5 μg/ml. Images were taken after 5 d of incubation. The solvent methanol was used as a control. The experiment was repeated three times with similar results. b Control effect on gray mold of apple and tomato caused by B.c Pyr-R5 using the bacterial suspension of the wild type strain ZJU23 fermented in KB (treatment KB&WT, before optimization), ΔLrhA fermented in HAI (treatment HAI&ΔLrhA, after optimization) or pyrimethanil (Pyr). Apple and tomato inoculated with B.c only was used as the non-treatment control. The representative images in each treatment were obtained after 3 d of treatment. c Lesion area and disease control efficacies of different treatments against gray mold disease caused by B.c Pyr-R5. Data presented are the mean ± s.e.m. from three biological replicates. Different letters indicate significantly different groups (P < 0.05, ANOVA and Fisher’s LSD test)

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