Fig. 4
PHBV accumulation and metabolites secreted. The PHA content respect to the cell dry weight (CDW) (A), the proportion of 3HV monomer respect to the total polymer (B) and the metabolites detected in the culture supernatant (C), the samples were measured at the late exponential phase where the maximum polymer accumulation is observed. Cells were grown in RRNCO medium (fructose 13.3 mM) with an initial OD660 of 0.05. Seven culture conditions were tested: six were grown in darkness, being three of them open systems (O1, O2, O3) and three closed systems (C1, C2, C3). A forth closed system in the presence of light was included in the experimental design. Data is expressed as mean ± SD of triplicate determinations obtained from three independent experiments