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Table 2 Strains used in this study

From: Loop pathways are responsible for tuning the accumulation of C19- and C22-sterol intermediates in the mycobacterial phytosterol degradation pathway

Strains

Description

Main product

Impurity

Refs.

HGMS2

Wild-type strain

4-AD (S5)

ADD (S6), 9OH-AD (S7)

[51]

HGMS2Δkstd1

Deletion of kstd211 from HGMS2

4-AD (S5)

9OH-AD (S7)

[52]

HGMS2Δkstd1/ΔkshA395

Deletion of kshA395 from HGMS2Δkstd1

4-AD (S5)

BA(S11)

[52]

HGMS6

Deletion of the kstd1, kshA395 and kshB122 genes from HGMS2, i.e., HGMS2Δkstd1/Δksh395/ΔkshB122

4-AD (S5)

BA(S11)

[52]

HGMS6ΔkshA226

Deletion of kshA226 from HGMS6

4-AD (S5)

BA(S11)

This study

HGMS6ΔkshA226/ΔopccR667

Deletion of both kshA226 and opccR667 from HGMS6

4-AD (S5)

BA(S11), Cpd1

This study

HGMS2Δhsd4A1

Deletion of hsd4A1 from HGMS2

BA (S11)

DBA

This study

HGMS2Δhsd42

Deletion of hsd4A2 from HGMS2

4-AD (S5)

ADD (S6)

This study

HGMS2ΔfadA5

Deletion of fadA5 from HGMS2

4-AD (S5)

BA (S11)

This study

HGMS2ΔhsaG

Deletion of hsaG from HGMS2

4-AD (S5)

ADD (S6)

This study

HGMS2Δkstd1/Δhsd4A1ΔhsaG

Deletion of hsaG from HGMS2Δkstd1/Δhsd4A1

BA (S11)

4-AD (S5)

This study

HGMS2Δkstd1/Δhsd4A1

Deletion of kstd1 from HGMS2Δhsd4A1

BA (S11)

4-AD (S5)

This study

HGMS2Δkstd1/Δhsd4A2

Deletion of kstd1 from HGMS2Δhsd42

4-AD (S5)

BA (S11)

This study

HGMS2Δkstd1/ΔfadA5

Deletion of kstd1 from HGMS2ΔfadA5

BA(S11)

Cpd2, 4-AD(S5)

This study

HGMS6Δhsd4A1

Deletion of hsd4A1 from HGMS6

BA(S11)

4-AD(S5)

This study

HGMS6Δhsd4A1/Δhsd4A2

Deletion of hsd4A2 from HGMS6Δhsd4A1

BA(S11)

4-AD(S5)

This study

HGMS7

Augmentation of pMV261-kstd2 in HGMS2Δhsd4A1

DBA

BA(S11)

This study

HGMS8

Augmentation of pMV261-kshA/B in HGMS2kstd1/hsd4A1

9OH-BA

4-AD(S5)

This study