Fig. 4

Comparison of the hsd4A-, hsaG-, fadA5- and kstd-knockout mutants for phytosterol fermentation in small-scale fermentation. a HPLC profiles of the extracts from phytosterol fermentation for 144 h in comparison with that of the HGMS2 strain. 1: HGMS2; 2: HGMS2^Δhsd4A1; 3: HGMS2^Δhsd4A2; 4: HGMS2^ΔhsaG; 5: HGMS2^ΔfadA5; 6: DBA and 7: 9OH-BA. Cpd2: an unidentified intermediate. Inset: TLC assay of the extracts from the fermentation broths with the HGMS2^Δhsd4A1mutant. Δhsd4A1#1 and Δhsd4A1#2 refer to two different colonies. b) Effect of kstd211-knockout on BA (S11) accumulation. 1. HGMS2; 2. HGMS2^{Δkstd1/Δhsd4A1}; 3. HGMS2^{Δkstd1/Δhsd4A2}; 4. HGMS2^{Δkstd1/ΔfadA5} and 5. HGMS2^{Δkstd1/hsdA1/ΔhsaG}