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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Loop pathways are responsible for tuning the accumulation of C19- and C22-sterol intermediates in the mycobacterial phytosterol degradation pathway

Fig. 1

Depleting 3-ketosteroid-9α-hydroxylases caused the accumulation of BA (S11) in the HGMS2 strain. a HPLC profiling of the extracts from the fermentation broths of different HGMS2 mutants in comparison with that of standard BA (S15). 1: HMGS2; 2: HMGS2Δkstd1/ΔkshA395; 3: HMGS6; 4: HMGS6ΔkshA226 and 5: BA (S15). b Mass spectra of the 4-AD (S5) and BA (S11) peaks collected from HPLC analysis

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