Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol

Fig. 5

The MHETase whole-cell catalyst is active across a range of pHs, temperatures, and over time. A MHETase activity at the indicated pH is plotted. MHETase activity was assayed with 26.8 μM MpNPT for 10 min at 24 °C, followed by measuring absorbance at 405 nm. Activity was normalized to a cell density of 108 cell/mL. Horizontal bars indicate the means of the replicates (n = 3). B MHETase activity at the indicated temperatures is plotted. n = 3. C Activity of purified MHETase at different temperatures is plotted. Purified enzyme was diluted to 2 nM and and assayed with 50 μM MpNPT. n = 3. D MHETase activity of the whole-cell catalysts was assayed at day 0, 4, and 12 during incubation at room temperature. At day 12, the cell suspension, cell pellet, and supernatants were assayed. MHETase activity was normalized to a cell density of 108 cell/mL at each day. n = 3. E Activity of purified MHETase over time. Purified enzyme was diluted to 2 nM and held at room temperature for 4 days. MHETase activity at day 4 was measured with 50 μM MpNPT at 25 °C alongside a fresh aliquot of purified MHETase (day 0). n = 2

Back to article page