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Table 1 Separation efficiency of the hanA1-EmGFP from 200 mL culture a

From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle

 

Total protein/mg

Purity/%b

Yield/mgc

Recovery/%d

Crude extract

27 ± 4

37 ± 5

10 ± 4

100

First-round separation

Second-round separation

15 ± 3

11 ± 2

57 ± 5

67 ± 4

8 ± 3

7 ± 1

83 ± 5

75 ± 4

  1. a Values are averages of experiments performed in triplicate ± standard error
  2. b Determined by densitometric analysis of Coomassie-stained SDS-PAGE
  3. c Yield = total protein × purity
  4. d Recovery is calculated as the residual yield divided by that from the crude extract
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Microbial Cell Factories

ISSN: 1475-2859

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