Fig. 6
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
SDS-PAGE analysis of the hanA1-EmGFP constructs via purification. A Heparin Sepharose purification. B Human S100A11 immobilized on amylose resin through the fused MBP tag at N-terminus for purification of the EmGFP construct. C Water-insoluble phosphatidylserine as the affinity matrix for purifying the protein. D Phenyl Sepharose purification. F Ni–NTA purification of His6-tagged hanA-EmGFP. In each figure, left: direct purification of the protein in soluble extracts. Right: purification of the protein via affinity separation. Lane 1: the washed proteins. Lane 2: the eluted proteins. Arrows indicated the fusion protein