Fig. 5
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Analysis of the added EDTA-Na2 on chromophores in two colored proteins. A SDS-PAGE analysis of purified His6-mSF. B UV–visible spectrum. The mSF was analyzed at room temperature on the spectrometer using a quartz cuvette sealed with a rubber septum. C SDS-PAGE analysis of purified His6-tagged hanA1-mSF. D UV–visible spectrum. The purified protein was incubated with 15 mM EDTA-Na2, exchanged with buffer A and analyzed at room temperature (black line). After purified protein was incubated with the compounds to reconstitute the Fe-S cluster under aerobic condition, the UV–visible spectrum (red line) was analyzed. E SDS-PAGE analysis of purified His6-tagged hanA1-Vhb. F UV–visible spectra of the purified hanA1-Vhb in the absence (black line) or the presence of Fe2+ (red line) treatment followed by exchange with buffer A. In this figure, lane 1: uninduced proteins. Lane 2: induced proteins. Lane 3: The eluted protein indicated by the arrow. The analyses were described in materials and methods section