Fig. 4
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Observation of the colored proteins separated by precipitating and re-solubilizing process. A The fluorescent proteins separation observed under UV light irradiation. Left: hanA1-EmGFP. Middle: hanA1-mCHerry. Right: hanA1-CrLOV. In Fig. 4 1: supernatant. 2: supernatant after CaCl2 precipitation. 3: supernatant with EDTA-Na2 redissolution. B The correspondent samples containing the fluorescent protein displayed under the visible light. C The colored proteins separation observed under visible light. Left: the hanA1-Vhb. Right: the hanA1-mSF. The purified His6-mSF containing the Fe-S cluster was indicated as C. D Fluorescence display of the mCherry (up), and CrLOV (down) with the hanA1 tag on the SDS-PAGE gel. E SDS-PAGE analysis of the hanA1-tagged mCherry (left) and CrLOV (right) after separation. F SDS-PAGE analysis of the hanA1-tagged Vhb (left) and mSF (right) after separation. In D and E, Lane 1: soluble fraction. Lane 2: the retained soluble proteins after precipitation. Lane 3: the re-solubilized proteins. Arrows indicated target proteins