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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle

Fig. 2

Separating the hanA1-EmGFP from the supernatant at 4 °C and 25 °C. A SDS-PAGE analysis of protein samples dissolved in buffer A after CaCl2 at different concentrations to precipitate the hanA1-EmGFP at 4 °C. B The same analysis for precipitating the fusion protein at 25 °C. C SDS-PAGE analysis of the supernatant after adding 10 mM CaCl2 for incubating various periods at 4 °C. D The same analysis of the correspondent samples for incubation at 25 °C. Arrows indicated the hanA1-EmGFP. E The fluorescence from clear lysates containing the fusion protein precipitated with 10 mM CaCl2 and stored at different periods at correspondent temperatures. The asterisk in this and other figures indicated significant differences lower than the clear lysate without precipitation as the control; * p < 0.01

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