Fig. 1
From: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
Overexpression and separation of the hanA1-EmGFP. A The confocal fluorescent micrographs for E. coli BL21(DE3) cells overexpressing the hanA1-EmGFP. Size bars, 10 μm. B Western bolt analysis of the induced EmGFP partner using anti-GFP antibodies. In all figures for SDS-PAGE and Western blot analyses, protein marker was labeled as “M”. Lane 1 represented the immunoblot analysis. The arrow indicated the hanA1-EmGFP. C SDS-PAGE analysis of the soluble and insoluble hanA1-EmGFP produced in E. coli BL21(DE3) cells. U: uninduced. I: induced. S: surpertant. P: pellet. D SDS-PAGE analysis of the extracts dissolved in buffer A with 100 mM NaCl substitution of 10% glycerol for precipitation. The final concentrations of added CaCl2 were denoted on the top of gels. E The correspondent samples dissolved with buffer A were also precipitated with the same treatment. All arrows indicated the bands representing the overexpressed fusion protein. F The retained fluorescence from extracts containing the fusion protein in absence and presence of 100 mM NaCl after precipitation treatment. The asterisk indicated significant differences lower than the clear lysate without precipitation as the control; * p < 0.01