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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Fig. 6

Time curves of fed-batch fermentation of γ-PGA in a 5 L fermenter by BL01 and BL01ΔpgdSΔggtΔsucAΔgudB:P43-citZ-pyk strain, a Cell growth curves, b γ-PGA growth curves, c Citric acid consumption curves, d Residual sugar curves. Data are given as the mean ± SD, n = 3. Aeration rate: 10 mL/min; agitation rate: 400 rpm (0–6 h), 500 rpm (6–12 h), 600 rpm (12–24 h), 700 rpm (24–48 h); the pH was controlled at pH 6.5 ± 0.2. 20 g of sodium citrate dissolved in 50 mL of sterile water was fed into the fermenter at 24 h, and 150 mL of feed medium containing 700 g/L of sucrose was fed at 30 and 36 h, individually

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