Effective gossypol removal from cottonseed meal through optimized solid-state fermentation by Bacillus coagulans

Table 1 The experimental results of Box-Behnken design and corresponding schemes

Treatment	Factor					Y₁ (10⁹ CFU/g wet material)	Y₂ (%)
Treatment	A	B	C	D	E	Y₁ (10⁹ CFU/g wet material)	Y₂ (%)
1	0.75	10	48	2	0.8	2.47	82.30
2	1.25	10	48	2	0.8	2.80	81.38
3	0.75	20	48	2	0.8	5.93	81.64
4	1.25	20	48	2	0.8	4.65	82.96
5	1.00	15	36	1	0.8	1.57	82.83
6	1.00	15	60	1	0.8	6.02	84.61
7	1.00	15	36	3	0.8	1.98	82.43
8	1.00	15	60	3	0.8	4.40	81.84
9	1.00	10	48	2	0.6	5.98	78.48
10	1.00	20	48	2	0.6	5.01	82.70
11	1.00	10	48	2	1.0	2.09	87.64
12	1.00	20	48	2	1.0	2.69	87.71
13	0.75	15	36	2	0.8	2.51	81.51
14	1.25	15	36	2	0.8	1.46	83.68
15	0.75	15	60	2	0.8	5.57	81.84
16	1.25	15	60	2	0.8	4.36	82.23
17	1.00	15	48	1	0.6	1.90	79.20
18	1.00	15	48	3	0.6	4.12	76.90
19	1.00	15	48	1	1.0	3.93	86.52
20	1.00	15	48	3	1.0	2.94	87.38
21	1.00	10	36	2	0.8	1.39	83.82
22	1.00	20	36	2	0.8	2.47	79.99
23	1.00	10	60	2	0.8	4.63	82.43
24	1.00	20	60	2	0.8	4.87	82.56
25	0.75	15	48	1	0.8	2.03	78.35
26	1.25	15	48	1	0.8	4.10	82.37
27	0.75	15	48	3	0.8	5.93	83.29
28	1.25	15	48	3	0.8	3.11	82.83
29	1.00	15	36	2	0.6	1.99	75.77
30	1.00	15	60	2	0.6	5.26	77.16
31	1.00	15	36	2	1.0	1.10	84.81
32	1.00	15	60	2	1.0	6.64	86.19
33	0.75	15	48	2	0.6	3.47	79.07
34	1.25	15	48	2	0.6	4.21	78.74
35	0.75	15	48	2	1.0	4.54	87.18
36	1.25	15	48	2	1.0	5.64	84.41
37	1.00	10	48	1	0.8	3.85	85.40
38	1.00	20	48	1	0.8	3.17	85.27
39	1.00	10	48	3	0.8	5.95	85.86
40	1.00	20	48	3	0.8	2.81	85.40
41	1.00	15	48	2	0.8	7.45	85.66
42	1.00	15	48	2	0.8	6.97	85.33
43	1.00	15	48	2	0.8	6.83	86.19

The concentrations of viable cells and detoxification efficiencies were selected as the response value Y₁ and Y₂. The independent variables A, B, C, D and E were corresponding to material to water, inoculation amount, incubation period, the content of expanded corn flour and FeSO₄ are shown in the table

ISSN: 1475-2859