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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Mining and characterization of the PKS–NRPS hybrid for epicoccamide A: a mannosylated tetramate derivative from Epicoccum sp. CPCC 400996

Fig. 3

Gene deletion of epiA and characterization of ΔepiA mutants. A Schematic representation of the epiA−disruption cassette with the genetic map and its surrounding regions. The dashed line represents expected size product for wild-type and deletant strains. B Diagnostic PCR characterization of ΔepiA mutants. Primer pair (Z05/Z06) located within the deletion region of epiA gene (Bi-613 bp in size for WT and no PCR amplicon for mutants); Primer pairs (Z07/Z12 and Z10/Z11) targeting the Hyg gene and epiA flanking regions (Bii-3256 bp and 3191 bp in size for mutants and no PCR amplicon for WT); Primer pairs (Z07/Z08 and Z09/Z10) located in the epiA deletion region and flanking regions (Biii-2741 bp and 2162 bp in size for WT and no PCR amplicon for mutants). C HPLC profiles of organic extracts obtained from the WT and ΔepiA mutants of Epicoccum sp. The positions of epicoccamide A are indicated in the chromatograms. The elution was monitored at 280 nm and shown on the same Y-scale. HPLC-MS analysis of ΔepiA metabolites in positive ion mode, EIC at m/z 556, [M-H]−.

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Microbial Cell Factories

ISSN: 1475-2859

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