Table 2 Concentrations of proteins (recombinant SHV-1 (protein 1)) extracted from 1 mL of cell suspensions collected at the last induction time nodes in three parallel small-scale expression trials
Culture temperatures | Induction time nodes | Soluble proteins (μg/mL) | Insoluble proteins (μg/mL) |
|---|---|---|---|
32 °C | 10 h | 3958 | 6200 |
27 °C | 15 h | 4316 | 4453 |
22 °C | 25 h | 8532 | 3582 |