Fig. 4From: Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vectorEffects of restriction sites on recombinant β-lactamase expression (A: recombinant SHV-1 (protein 1 and 2) expression using BamHI/EcoRI and NcoI/XhoI as restriction sites, respectively; B: recombinant TEM-1 (protein 1 and 2) expression using BamHI/EcoRI and NcoI/XhoI as restriction sites, respectively; and C: the protein 3 (recombinant SHV-1 and TEM-1) expression using NdeI/XhoI as restriction sites)Back to article page