Fig. 3From: Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vectorEffects of IPTG concentration (A), culture temperature (B–E), and induction time (C–E) on recombinant SHV-1 (protein 1) expression (The same results were also obtained in the TEM-1 heterologous expression)Back to article page