Fig. 2From: Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vectorGrowth curves of host 1 cells separately cultivated at 32 (A), 27 (B), and 22 °C (C) in small-scale expression trials (blue-marked: IPTG added to the culture systems; red-marked: induction time nodes)Back to article page