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Table 3 Comparison with previous reports of bread to ethanol process including SSF and SHF

From: Simultaneous saccharification and ethanologenic fermentation (SSF) of waste bread by an amylolytic Parageobacillus thermoglucosidasius strain TM333

Bread type

Treatment/fermentation conditions

Bread concentration % w/w

**Ethanol yield per kg of bread @ 48 h

Enzymes used

References

Waste wheat-rye bread

SSF (slurried with enzymes at 35 °C prior to fermentation)

15

333.31 (*354.36)

α-Amylase and glucoamylase, plus protease

[45]

Waste wheat-rye bread

SSF (slurried at 45 °C for 20 min prior to fermentation)

15

373.15 (*398.4)

α-Amylase, β-glucanase, pentosanase, cellulase, plus protease

[45]

Waste wheat-rye bread

SSF of liquefied WB

30

416–425

α-Amylase, amyloglucosidase plus protease

[46]

Waste wheat-rye bread

SSF post microwave pretreatment

15

375.5 (*384.6)

α-Amylase and glucoamylase, plus protease

[45]

Waste wheat-rye bread

SSF post Ultrasonic pretreatment

15

365.1 (*366.8)

α-Amylase and glucoamylase, plus protease

[45]

Waste wheat-rye bread

Separate hydrolysis and fermentation (SHF) standard process

15

378.7 (*386.0)

STARGEN 002 (α-amylase and glucoamylase) plus protease

[45]

mixture of wheat and buckwheat flours

SHF standard process

16

303.2–408.1 (67 h)

α-Amylase plus a glucoamylase

[1]

Sandwich WB

Yeast SSF of gelatinized and/liquefied WB

3

456–478.4

(*466–474.3)

α-Amylase plus a glucoamylase

This work

Sandwich WB

Yeast SSF of untreated WB

3

448.9 (*459.9)

α-Amylase plus a glucoamylase

This work

Sandwich WB

Thermophilic bacteria SSF of untreated WB

3

437.1 (*446.8)

P. thermoglucosidasius TM333 crude α-amylase only

This work