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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhancement of acarbose production by genetic engineering and fed-batch fermentation strategy in Actinoplanes sp. SIPI12-34

Fig. 2

Overexpression of TetR1 to increase acarbose production. a Acarbose production profiles of SIPI12-34 and SIPI12-34/TetR1 were analyzed by HPLC. SIPI12-34/TetR1: SIPI12-34 overexpressing the TetR1. Error bars show standard deviations, ***P < 0.001. b Transcriptional analysis of acarbose BGC in SIPI12-34 and SIPI12-34/TetR1 during the fermentation process by quantitative real-time PCR. RNA samples were isolated from SIPI12-34 and SIPI12-34/TetR1 at 48 h and 72 h respectively, in shake flask culture. At each time point, the expression levels of each transcriptional unit in SIPI12-34 were taken as 1 to quantify the relative expression levels in SIPI12-34/TetR1, respectively. Each strain has 3 replicates at different time points

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