Table 1 List of E. coli strains and plasmids used in this study
Strains and plasmids | Genotype—descriptions | Reference |
|---|---|---|
Strains | ||
E. coli Trans1-T1 | F-φ80(lacZ)ΔlacX74hsdR (rk−, mk−) ΔrecA 1398endA 1 tonA | Transgen |
E. coli NT1003 | L-lysine hyper-producing strain | [6] |
E. coli BL21-1-gfp | E. coli BL21(DE3), pRL1:gfp. Kan | This work |
E. coli BL21-2-gfp | E. coli BL21(DE3), pRL2:gfp. Kan | This work |
E. coli BL21-3-gfp | E. coli BL21(DE3), pRL3:gfp. Kan | This work |
E. coli BL21-4-gfp | E. coli BL21(DE3), pRL4:gfp. Kan | This work |
E. coli NT3201 | E. coli NT1003ΔldhA | This work |
E. coli NT3202 | E. coli NT1003ΔackA | This work |
E. coli NT3203 | E. coli NT1003ΔldhAΔackA | This work |
E. coli KAC | E. coli NT3203, Ptrc:cadA. Kan | This work |
E. coli KARL3 | E. coli NT3203, PRL3:cadA. Kan | This work |
E. coli KARL4 | E. coli NT3203, PRL4:cadA. Kan | This work |
Plasmids | ||
pET28a-gfp | Cloning gfp gene into BamH I and Hind III site of pET28a | |
pRL1-gfp | One-step cloning pRL1 promoter to replace T7 promoter and lac operator in pET28a-gfp | This work |
pRL2-gfp | One-step cloning pRL2 promoter to replace T7 promoter and lac operator in pET28a-gfp | This work |
pRL3-gfp | One-step cloning pRL3 promoter to replace T7 promoter and lac operator in pET28a-gfp | This work |
pRL4-gfp | One-step cloning pRL4 promoter to replace T7 promoter and lac operator in pET28a-gfp | This work |
pRL3-cadA | One-step cloning cadA gene to replace gfp gene in pRL3-gfp | This work |
pRL4-cadA | One-step cloning cadA gene to replace gfp gene in pRL4-gfp | This work |
Ptrc-cadA | One-step cloning ptrc promoter to replace pRL3 promoter in pRL3-cadA | This work |
sgRNA-ΔldhA | Vector for deletion of ldhA gene of the E. coli; Smr | This work |
sgRNA-ΔackA | Vector for deletion of ackA gene of the E. coli; Smr | This work |