Table 3 Transcriptional changes of genes involved in the metabolism of acetyl CoA and PHB synthesis in cbbR and regA-overexpressing strain
Metabolism | Gene | Description | Fold change |
|---|---|---|---|
Acetyl-CoA related metabolism | acs (H16_A1616) | Acetyl-coA synthetase; ATP + Acetate + CoA ↔ AMP + Diphosphate + Acetyl-CoA | 0.34 |
pta1 (H16_B1631) | phosphate acetyltransferase; acetyl-CoA → acetate | 0.44 | |
H16_B1102 | acetyl-CoA synthetase; ATP + Acetate + CoA ↔ AMP + Diphosphate + Acetyl-CoA | 0.28 | |
ackA (H16_B1630) | Phosphate acetyltransferase-acetate kinase pathway, acetyl-CoA → acetate | 0.63 | |
ackA2 (H16_A0670) | Phosphate acetyltransferase-acetate kinase pathway, acetyl-CoA → acetate | 0.65 | |
PHB synthesis | phaA (H16_A1438) | acetyl-CoA acetyltransferase | 2.2 |
phaB1 (H16_A1439) | acetoacetyl-CoA reductase | 1.7 | |
phaC2 (H16_A2003) | polymerase subunit PhaC | 1.6 | |
phaZ1 (H16_A1150 | PHB depolymerase | 0.27 | |
phaZ2 (H16_A2862) | PHB depolymerase | 2.0 |