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Table 1 Strains and plasmids used in this work

From: Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO2

Strains

Description

Source

C. necator H16

Wild type gentamycin resistant (Genr)

ACTC 17699, KCTC 22469

CbbR

C. necator H16 carrying pCbbR

This study

RegA

C. necator H16 carrying pRegA

This study

CbbR-RegA

C. necator H16 carrying pCbbR-RegA

This study

RbcLXS

C. necator H16 carrying pRbcLXS

This study

Plasmids

 pBBR1MCS2

Broad host range plasmid; PLac, Kmr

Addgene

 pBAD

pBBR1MCS2-derived expression vector containing L-arabinose inducible araBAD promoter and araC; Kmr

This study

 pCbbR

pBAD with cbbR; Kmr

This study

 pRegA

pBAD with regA; Kmr

 

 pCbbR-RegA

pBAD with cbbR-RBS-regA; Kmr

This study

 pRbcLXS

pBAD with rbcL-RBS-rbcX-RBS-rbcS; Kmr

This study