Table 5 Organic acid distribution for shake flask cultivations of A. oryzae with different initial product concentrations
Conditiona | Organic acid distribution [%]b | ||||||
|---|---|---|---|---|---|---|---|
Malate | Succinate | Fumarate | Pyruvate | α-Ketoglutarate | Oxalate | Citrate | |
2 g/L M | 50.1 ± 1.3 | 40.0 ± 2.7 | 6.1 ± 0.5 | 0.9 ± 0.1 | 0.3 ± 0.0 | 1.7 ± 0.5 | 0.9 ± 0.4 |
6 g/L M | 46.7 ± 0.9 | 38.4 ± 1.9 | 10.8 ± 0.4 | 0.8 ± 0.0 | 0.5 ± 0.1 | 1.9 ± 0.5 | 1.0 ± 0.5 |
10 g/L M | 42.3 ± 1.6 | 35.3 ± 1.5 | 15.6 ± 0.5 | 0.9 ± 0.1 | 0.5 ± 0.1 | 3.9 ± 0.7 | 1.5 ± 0.8 |
20 g/L M | 36.0 ± 3.4 | 34.6 ± 2.6 | 23.3 ± 3.6 | 1.0 ± 0.2 | 0.4 ± 0.1 | 3.7 ± 2.2 | 1.0 ± 0.3 |
35 g/L M | 26.9 ± 1.6 | 35.3 ± 1.9 | 34.1 ± 1.7 | 1.0 ± 0.1 | 0.3 ± 0.0 | 2.0 ± 0.7 | 0.3 ± 0.3 |
50 g/L M | 16.6 ± 3.0 | 35.1 ± 1.6 | 45.5 ± 4.2 | 0.9 ± 0.1 | 0.3 ± 0.0 | 1.6 ± 0.5 | 0.0 ± 0.0 |
1 + 1 g/L M + S | 52.2 ± 1.6 | 40.2 ± 3.0 | 4.6 ± 0.3 | 0.7 ± 0.0 | 0.3 ± 0.1 | 1.5 ± 0.8 | 0.4 ± 0.2 |
3 + 3 g/L M + S | 51.9 ± 0.6 | 34.3 ± 2.4 | 8.3 ± 0.5 | 0.8 ± 0.2 | 0.4 ± 0.1 | 3.3 ± 2.3 | 1.0 ± 0.1 |
5 + 5 g/L M + S | 50.3 ± 2.6 | 34.7 ± 6.8 | 10.3 ± 1.2 | 0.9 ± 0.1 | 0.5 ± 0.1 | 2.8 ± 2.7 | 0.7 ± 0.4 |
10 + 10 g/L M + S | 44.0 ± 0.5 | 40.0 ± 0.6 | 12.3 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0.0 | 1.5 ± 0.3 | 1.0 ± 0.1 |
17.5 + 17.5 g/L M + S | 37.8 ± 0.8 | 38.9 ± 2.0 | 18.3 ± 0.7 | 0.8 ± 0.1 | 0.3 ± 0.0 | 3.2 ± 2.4 | 0.7 ± 0.1 |
25 + 25 g/L M + S | 32.9 ± 1.6 | 39.6 ± 0.9 | 23.1 ± 1.5 | 0.8 ± 0.1 | 0.3 ± 0.0 | 2.7 ± 0.7 | 0.5 ± 0.1 |
Control | 53.1 ± 0.5 | 37.4 ± 0.6 | 4.0 ± 0.1 | 0.7 ± 0.0 | 0.5 ± 0.0 | 2.8 ± 1.0 | 1.4 ± 0.2 |