From: Optimization of l-malic acid production from acetate with Aspergillus oryzae DSM 1863 using a pH-coupled feeding strategy
Fermentation No.
CaCO3 [g/L]
pH correction
I
90
–
II
50
III
10 M acetic acid
IV
10 M acetic acid (pH 3.0)
V
10 M acetic acid (pH 4.5)
VI
VII
90 + 42 g after 168 h
10 M acetic acid, 5 M NaOH