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Table 1 Summary of cultivation conditions evaluated in 2.5-L bioreactors with a working volume of 1.4 L

From: Optimization of l-malic acid production from acetate with Aspergillus oryzae DSM 1863 using a pH-coupled feeding strategy

Fermentation No.

CaCO3 [g/L]

pH correction

I

90

II

50

III

50

10 M acetic acid

IV

50

10 M acetic acid (pH 3.0)

V

50

10 M acetic acid (pH 4.5)

VI

90

10 M acetic acid

VII

90 + 42 g after 168 h

10 M acetic acid, 5 M NaOH

  1. Stirrer velocity was 600 rpm, aeration 0.5 vvm and temperature 32 °C for all fermentations