Segregationally stabilised plasmids improve production of commodity chemicals in glucose-limited continuous fermentation

Table 2 The calculated half-lives based on a regression fit of the RFP + percentage retention data, the time at which half of the cells no longer exhibit any fluorescence expression and are therefore likely to have lost plasmid, in a continuous fermentation at a dilution rate of 0.1 h⁻¹

	pQR2401 (cer −)	pQR2402(cer +)
Half life (hours)	44.632	70.332
Overall productivity (g.L⁻¹.h⁻¹)	0.073	0.12
Peak productivity (g.L⁻¹.h⁻¹)	0.24	0.19
Product yield (g_citramalate.g_glucose⁻¹)	0.13	0.21

The overall productivity across the entire fermentation and the peak productivity at the maximal citramalate productive period are shown as grams of product produced, per litre of fermentation broth, per hour. The product yield is shown as the amount of citramalate produced compared to the glucose added throughout the length of the fermentation