Fig. 3
HPLC data showing the concentration of citramalate and glucose (primary axis), and OD600nm (Secondary axis) for cells containing either plasmid pQR2401 (a) or pQR2402 (b). Graphs show glucose concentration, in blue, squares; citramalate concentration, in red, squares; and OD in orange, triangles. Samples were taken at intervals throughout a single continuous fermentation and concentration of analyte was determined by HPLC. A plot of bulk fluorescence values (c) shows the fluorescence signal from FresnoRFP for samples containing cells with either plasmid pQR2401 (blue circles) or plasmid pQR2402 (red circles) from the corresponding fermentation, determined from the excitation/emission (545/592) in a plate reader spectrometer