Fig. 3

Confirmation of the genome-engineered E. coli stains by de novo genome sequencing. A, B Circle plots and partial enlarged views of genome-engineered E. coli strains. C The formation process in which Chr.4.64M^Mut was reassembled from Chr.2.28 M and Chr.2.36 M by a homologous recombination based on H, confirmed by the sequencing results, and comparison with the Chr.4.64M^WT genome