Fig. 3
From: Origin of the 3-methylglutaryl moiety in caprazamycin biosynthesis
Extracted ion chromatograms from extracts of S. coelicolor M1154 mutants with either sco2774, sco2779 or both deleted and harboring a caprazamycin gene cluster containing a cpz5 deletion. Retention time from 12 to 16Â min is shown. Captions indicate mutant genotypes. Highlighted are signals for hydroxyacylcaprazols E/F with m/z of 802.5 and caprazamycin aglycons E/F with m/z of 930.5 acquired in positive mode. Non-highlighted signals represent sulfated hydroxyacylcaprazols E/F or sulfated caprazamycin aglycons E/F respectively. Extract of a culture supplemented with 3-methylcrotonyl-SNAc is shown in blue