Fig. 7
From: Garvicin Q: characterization of biosynthesis and mode of action
Heterologous expression of garQICDCgl in C. glutamicum. A Optical density (OD600; solid lines) and pH (broken lines) in supernatants of C. glutamicum CR099/pPBEx2-garQICDCgl cultivated in CGXII + 2% glucose with (red lines) or without (blue lines) urea. B antimicrobial activity in SNs of these cultivations sampled at the indicated timepoints. Activity was assayed using L. lactis IL1403/pNZ-pHin2Lm as indicator. Values are ratios of fluorescence intensity at 520 nm after excitation at 400 and 480 nm (ratio RFU 400/480) and are mean of n = 3 independent experiments (supernatants of independent cultivations). C Purification of garvicin Q from SN of C. glutamicum CR099/pPBEx2-garQICDCgl after cultivation for 24 h in CGXII medium + 2% glucose without urea by hydrophobic interaction chromatography (HIC; upper panel) and subsequent reverse-phase chromatography (RPC; lower panel). D LC–MS analysis of pooled RPC elution fractions harbouring antimicrobial activity. The peak with a mass to charge ratio (m/z) of 1069.2010 corresponds to garvicin Q with five positive charges